Abstract
We have studied the role of the membrane-associated form of IL-1 on bone resorption in vitro. Murine macrophages of the P388D1 cell line stimulated with LPS, subsequently fixed with paraformaldehyde, induced the proliferation of C3H/HeJ mouse thymocytes in the presence of a submitogenic concentration of Con A. Membrane IL-1 on P388D1 cells stimulated with LPS induced bone resorption in organ cultures of neonatal BALB/c mouse calvaria. Polyclonal antibodies directed against membrane IL-1 and soluble IL-1 from P388D1, and monospecific rabbit anti-murine rIL-1 alpha serum neutralized the membrane IL-1 activity, as measured by the thymocyte proliferation. In addition, these antibodies suppressed the bone resorption induced by membrane IL-1. The bone resorption induced by membrane IL-1 required direct contact between mouse calvaria and membrane IL-1. Salmon calcitonin strongly suppressed the calcium release from mouse calvaria in the presence of membrane IL-1. Indomethacin partially inhibited the bone resorption induced by membrane IL-1 on P388D1 cells. Moreover, membrane IL-1 on LPS-stimulated BALB/c mouse peritoneal macrophages or LPS-stimulated osteoblastic cells from BALB/c mouse calvaria induced bone resorption in vitro. These results suggest that membrane IL-1 on macrophages and osteoblastic cells may have a significant role in inflammatory bone resorption in vivo.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
