Abstract
Nuclear envelope assembly is an essential event in cell cycle but its mechanism and regulation remain mostly unknown. Using a cell-free system derived from sea urchin gametes we report that nuclear envelope formation involves the fusion of membrane vesicles highly enriched in phosphoinositides via the production of a fusogenic lipid, the diacylglycerol. By performing time course fluorescence lifetime imagery, we measured the kinetic of this process and demonstrate that nuclear envelope assembly is polarised. It is initiated at the poles of the nucleus where the nuclear envelope remnants are located. This study provides a mechanism for temporal control of NE assembly and offers an explanation for how such a process of membrane fusion can be spatially regulated.
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