Abstract
Transplantation of primary GRSL cells in the ascitic form led to a decrease in membrane microviscosity as measured by the fluorescence polarization technique. The transplanted GRSL ascitic cells showed a markedly lower ability to form caps with respect to both virus-related (MLr, GIX) and normal (H-2.7(G), H-2.8(K) and TL1.2) cell-surface antigens and their appropriate antisera in the indirect membrane immunofluorescence tests, than did primary GRSL cells, transplanted GRSL cells growing in solid form, and thymocytes, which all exhibited significantly higher membrane microviscosities. Transplantation of primary GRSL cells into syngeneic mice pre-irradiated with 400 rad did not lead to a fall in membrane microviscosity. It is suggested that the host immune response in intact mice leads to a selective survival of ascitic tumour cells with low membrane microviscosity.
Highlights
Summary.-Transplantation of primary GRSL cells in the ascitic form led to a decrease in membrane microviscosity as measured by the fluorescence polarization technique
In the Bentvelzen, 1978) and a low-leukaemia present investigation, we observed a relationship between capping ability and microviscosity of membrane lipids of these cells, as measured by the fluorescence polarization technique using the fluorophore 1,6-diphenyl-1,3,5,hexatriene(DPH) as a probe
The fluorescence polarization technique using DPH is frequently used as a probe for measuring membrane lipid fluidity in intact cells, isolated plasma membranes, purified enveloped virus (Barenholz, Moore and Wagner, 1976) and lipid liposomes (Shinitzky and Inbar, 1974; Van Blitterswijk et al, 1977; Barenholz et al, 1976; Collard et al, 1977)
Summary
Summary.-Transplantation of primary GRSL cells in the ascitic form led to a decrease in membrane microviscosity as measured by the fluorescence polarization technique. The transplanted GRSL ascitic cells showed a markedly lower ability to form caps with respect to both virus-related (MLr, GIx) and normal (H-2.7(G), H-2.8(K) and TL1.2) cell-surface antigens and their appropriate antisera in the indirect membrane immunofluorescence tests, than did primary GRSL cells, transplanted GRSL cells growing in solid form, and thymocytes, which all exhibited significantly higher membrane microviscosities. Transplantation of primary GRSL cells into syngeneic mice pre-irradiated with 400 rad did not lead to a fall in membrane microviscosity. Nicolson (1972) is compatible with the mobility of cell-surface antigens, both viral translateral mobility of cell-surface recep- and normal, under cross-linking conditors. The capping of surface antigens on GRS/A (GR) mouse thymocytes and thymusderived leukaemia (GRSL) cells as a
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