Abstract
Physiological changes of Lactobacillus plantarum (KFRI 815) by high pressure CO 2 treatment were investigated to examine the relevance to microbial inactivation. Characteristic properties of the cells measured in this study included salt tolerance, release of UV-absorbing substances, Mg and K ions, proton permeability, glycolysis, H +-ATPase and constitutive enzymes, and dye uptake. The cells treated with high pressure CO 2 of 7 MPa at 30°C for 10 min showed the irreversible cellular membrane damages including loss of salt tolerance, leakage of UV-absorbing substances, release of intracellular ions, collapse of proton permeability and uptake of Phloxine B dye. L. plantarum cells after CO 2 treatment also exhibited reduced glycolytic activity and inactivation of some constituent enzymes. However, H +-ATPase of the cell membrane maintained its initial specific activity of about 2.50 U/mg protein even though viability of the cells was reduced by several log cycles after high pressure CO 2 treatment.
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