Membrane current noise in toad retinal rods exposed to low external calcium.

Abstract

Outer segment membrane current of single rod photoreceptors from toad retina was recorded with a suction electrode, and extracellular calcium concentration was manipulated by transferring the recorded cell from one pool of saline to another or by locally perfusing the outer segment. The large increase in dark current that resulted from exposure to low-calcium saline was accompanied by an increase in dark noise in the band 1-800 Hz. This noise was suppressed by bright light, and its power spectrum could be described by a single Lorentzian equation with average corner frequency of 40.1 +/- 9.5 Hz (mean +/- S.D., n = 11). In low-calcium saline, saturating flash responses were often followed by a transient increase in the dark current lasting 30-100 s. During this rebound period of increased dark current, increased dark noise similar to that described in 2 was observed. The power spectrum of this noise was also fitted by a single Lorentzian equation, with corner frequency averaging 29.7 +/- 6.6 Hz (mean +/- S.D., n = 27). To examine the possible role of intracellular voltage fluctuations in generating the noise, suction electrodes were filled with calcium-free saline and recordings were made from outer segments of rods attached to pieces of retina. In this recording configuration, the electrical coupling among the rods in the piece should attenuate voltage fluctuations associated with the post-light rebound period of increased dark current. In this situation, the rebound increase in dark current was still observed, but the noise was reduced or absent. Using the same recording configuration, isolated rods showed pronounced noise during the rebound. The result in 4 suggests that the noise resulted from fluctuations in intracellular voltage, not directly from fluctuations in the light-sensitive channels. In this view, the corner frequency of the noise power spectrum probably reflects the membrane time constant of the isolated rod.