Membrane-bound progestin receptors in channel catfish and zebrafish ovary: Changes in gene expression associated with the reproductive cycles and hormonal reagents

Abstract

Membrane-bound progestin receptors (mPRs) are potential intermediaries in meiotic maturation of fish oocytes and other physiological processes. In this study, gene expression of the mPRs in the ovary of catfish and zebrafish during the reproductive cycle and the hormonal regulation of the expression were investigated. The transcript abundance of catfish mPRα gradually increased in conjunction with ovarian growth and then decreased prior to spawning period whereas the ovarian gene expression of mPRβ varied little throughout the reproductive cycle. In contrast, mPRγ gene expression peaked early in the mid-vitellogenic stage. The transcript abundance of zebrafish mPRα and β was low in ovarian follicles at early stages of oogenesis and gradually increased after the onset of vitellogenic growth and, thereafter, the gene expression did not vary. Gonadotropic treatment did not modulate the ovarian expression of mPRα and β genes in either catfish or zebrafish. On the other hand, exposure to 17,20β-dihydroxy-4-pregenen-3-one (the maturation-inducing steroid in this species) resulted in the down-regulation of mPRα in catfish ovary whereas gene expression was significantly induced by estradiol-17β. Taken together, these findings suggest that gonadotropin-induced final oocyte maturation may not require an induction of mPR(s) expression or that the gonadotropin stimulates mPR protein production at the post-transcriptional level, presuming these receptors are indispensable for oocyte maturation.