Abstract
Dissimilatory nitrite reductase (Nir) of the fungus Cylindrocarpon tonkinense was isolated and partially characterized. Nir activity was recovered in both the soluble and the membrane fractions, giving a specific activity of 4.74 and 5.80 μmol NO min–1 (mg protein)–1, respectively. The soluble and membrane-associated Nir preparations resembled each other in properties, and the results suggested that Nir is a homodimer of a 67-kDa subunit. The absorption spectrum and the inhibition by diethyldithiocarbamate indicated that fungal Nir is a copper-containing Nir (CuNir).
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