Melting point depression of Piroxicam in carbon dioxide + co-solvent mixtures and inclusion complex formation with β-cyclodextrin

Abstract

This paper reports on the melting point depression and polymorphic transformations of Piroxicam in supercritical carbon dioxide and in mixtures with ethanol, acetone or ethyl acetate as co-solvents. It further reports on the formation of the inclusion complexes of Piroxicam which is liquefied in these fluid mixtures with β-cyclodextrin. It is shown that in carbon dioxide the melting temperature of Piroxicam is reduced by 17°C, from 200°C to 183°C. The melting temperature was further depressed in mixtures containing a small amount of a co-solvent. The largest melting point depression was 37°C and was observed in the fluid mixture containing 10wt% ethanol. Piroxicam that was melted and recrystallized in carbon dioxide or carbon dioxide+co-solvent mixtures displayed distinct differences in its infrared spectra, thermal behavior and X-ray diffraction patterns as compared to its unprocessed form. Melting in CO2 and CO2+co-solvent mixtures was found to lead to a polymorphic transformation of Piroxicam from the β crystal form (cubic) to the α crystal form (needle). It was further found that melting of Piroxicam in 90:10wt% CO2:ethanol fluid mixture promotes inclusion complex formation with β-cyclodextrin leading to a 1:1 molar ratio complex. Inclusion complex formation via melting in supercritical fluid mixtures is now being proposed as a new processing methodology.