MELICA UNIFLORA YAPRAKLARININ ETANOLİK ÖZÜTLERİNİN IN VITRO BİYOLOJİK AKTİVİTESİNİN DEĞERLENDİRİLMESİ

Abstract

Melica uniflora is part of the omnivore and herbivore animal diets and there is not enough study about this plant. The aim of the study is to provide the antimicrobial and antioxidant potential of ethanol extract of M. uniflora leaves. Total phenolic and flavonoid contents and antioxidant potential based on different methods and antimicrobial activity of M. uniflora leaves ethanol extract were examined. Disk diffusion method against to eight bacteria strains, the Folin-Ciocalteu method, DPPH free radical scavenging activity and ferric reducing antioxidant power assays were used. Mean diameters of inhibition zones were found in the range of 14.93 mm to 26.32 mm. The total phenolic content of ethanol extract of M. uniflora leaves was determined as 0.466 mg GAE/g. Total antioxidant activity value of the extract was calculated 4.54 mg AAE/g. IC50 value was calculated 18.798 mg/mL for DPPH free radical scavenging activity. The FRAP value indicated that the reducing power of 1 gram of sample was equivalent to 3.33 µmol of trolox. The flavonoid content of the extract was calculated as 4.44 mgQE/g. According to the obtained results, the analyzed M. uniflora leaves ethanol extracts demonstrated antioxidant and antimicrobial activity level can be considered effective.