Abstract
The production of reactive oxygen species (ROS) during cryopreservation process impairs the sperm characteristics and fertilizing ability. However, melatonin, an antioxidant, could protect spermatozoa against this cell damage during cryopreservation. Therefore, we attempted to evaluate whether the melatonin supplementing in the semen extender could improve the sperm quality of swamp buffalo during cryopreservation. The semen collected from six swamp buffalo bulls were diluted with tris-citrate egg yolk extender supplementing with 0, 0.1, 0.5, 1.0, 2.0 and 3.0mM of melatonin. The parameters of sperm viability and motility were evaluated using computer-assisted semen analyser (CASA) after cryopreservation on days 1, 7, 15 and 30. The group supplemented with 1.0mM melatonin exhibited the higher viability after cryopreservation on days 1, 7, 15 and 30 with 58.346±2.1a , 57.586±2.0a , 55.082±1.8a and 55.714±1.8a , respectively, and showed the best results of motility parameters. However, higher concentration of melatonin at 3.0mM impaired all the parameters. In conclusion, the addition of melatonin at 1mM to semen extender could exert the best protection against sperm damage in swamp buffalo bull during cryopreservation.
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