Melatonin Regulates Root Meristem by Repressing Auxin Synthesis and Polar Auxin Transport in Arabidopsis.

Qiannan Wang,Haitao Shi,Bang An,Yunxie Wei,Chaozu He,Hongli Luo,Russel J Reiter

doi:10.3389/fpls.2016.01882

Abstract

Melatonin (N-acetyl-5-methoxytryptamine) plays important roles in regulating both biotic and abiotic stress tolerance, biological rhythms, plant growth and development. Sharing the same substrate (tryptophan) for the biosynthesis, melatonin and auxin also have similar effects in plant development. However, the specific function of melatonin in modulating plant root growth and the relationship between melatonin and auxin as well as underlying mechanisms are still unclear. In this study, we found high concentration of melatonin remarkably inhibited root growth in Arabidopsis by reducing root meristem size. Further studies showed that melatonin negatively regulated auxin biosynthesis, the expression of PINFORMED (PIN) proteins as well as auxin response in Arabidopsis. Moreover, the root growth of the triple mutant pin1pin3pin7 was more tolerant than that of wild-type in response to melatonin treatment, suggesting the essential role of PIN1/3/7 in melatonin-mediated root growth. Combination treatment of melatonin and 5-Triiodobenzoic acid (TIBA) did not enhance melatonin-mediated reduction of root meristem size, indicating that polar auxin transport (PAT) may be necessary for the regulation of root meristem size by melatonin treatment. Taken together, this study indicates that melatonin regulates root growth in Arabidopsis, through auxin synthesis and polar auxin transport, at least partially.

Highlights

Melatonin (N-acetyl-5-methoxytryptamine), a widely distributed endogenous bio-molecule in mammals, was first discovered in the bovine pineal gland in 1958 (Lerner et al, 1958)
3-day-old seedlings were kept growing under different concentrations of melatonin for another 6 days, and we found that both the number of meristem cells and the length of meristem are significantly reduced with increased concentration of melatonin (Figures 2B,C), indicating that melatonin-mediated repression of primary root growth might be due to reduced root meristem
YUCCA (YUC) proteins, TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS (TAA) family, TAA RELATED 1 and 2 play important roles in auxin (IAA) biosynthesis during plant development (Cheng et al, 2006; Yamamoto et al, 2007; Stepanova et al, 2008; Tao et al, 2008), so we investigated the effects of melatonin on the transcript levels of these genes (Figure 3A)

Summary

Introduction

Melatonin (N-acetyl-5-methoxytryptamine), a widely distributed endogenous bio-molecule in mammals, was first discovered in the bovine pineal gland in 1958 (Lerner et al, 1958). Numerous studies have shown that melatonin is widely involved in regulating both the biotic and abiotic stress tolerance, biological rhythms, plant growth and development Melatonin is synthesized from tryptophan as substrate by four key enzymes [tryptophan decarboxylase (TDC), tryptamine 5-hydroxylase (T5H), serotonin N-acetyltransferase (SNAT), and N-acetylserotonin O-methyltransferase (ASMT)] (Kang et al, 2007a,b, 2011, 2013; Okazaki et al, 2009; Fujiwara et al, 2010; Byeon and Back, 2014b, 2015; Zuo et al, 2014; Arnao and Hernández-Ruiz, 2015). There are two different pathways for the synthesis of auxin (IAA) in plants, one is tryptophan-independent, and the other is tryptophan-dependent, sharing the same substrate with melatonin (Wang et al, 2015). Tryptophandependent pathway is dependent on precursor tryptophan, through tryptamine (TAM), indole-3-pyruvic acid (IPyA) and indole-3-acetaldoxime (IAOx) pathways (Benjamins and Scheres, 2008; Strader and Bartel, 2008; Chandler, 2009)

Methods

Results

Conclusion