Melatonin Loaded Biodegradable Nano-Particles and Osteoporosis: A Mini Review

Abstract

Melatonin loaded PLA-Nano particles are nowadays important in biological system for it’s biodegradable nature for targetted drug delivery. Hence, aim of the study is to note applicability and toxicity of Mel-PLA-Nano particles in osteoprosis. Different concentrations of melatonin and PLA were prepared by dissolving in dichloromethane (DCM). The final dried nano-paticles were used for structural analysis by SEM, TEM, FTIR. Toxicity and immunological impact of nano-particles were evaluated on rats; control and nano-particle treated, (n=5/group) for 7 days. Afterwards animals were sacrificed and blood, liver and kidney were collected. A fraction of blood was processed for TLC, DLC and % LC, remaining was centrifuged at 3000x g at 4°C for 30 min. Seperated plasma was used for measurements of IL-2, IL-6, TNF-α, IFN-γ, IL-1β, urea, creatinine and BUN. Both plasma and tissue homogenates were used for AST, ALT, ACP, ALP estimations. We noted signifacntly high (p < 0.05) levels of TLC, DLC, %LC and IL-2, TNF-α upon treatment. Rest of the parametrs were found to be significantly low (IL-6, IL-1β, AST, ALT; p > 0.05) or un-affected (IFN-γ, ACP and ALP). From our preliminary study we may conclude that we have synthesized Mel-PLA-nano-particles and their effects were non-toxic to animals.