Melatonin induces follicle maturation in Danio rerio.

Oliana Carnevali,Francesca Maradonna,Giorgia Gioacchini,Beatrice Migliarini,Ike Olivotto,Immo A Hansen

doi:10.1371/journal.pone.0019978

Oliana Carnevali, Francesca Maradonna + Show 4 more

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https://doi.org/10.1371/journal.pone.0019978

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Abstract

Most organisms modulate their reproductive activity responding to day length by the nocturnal release of melatonin by the pineal gland. This hormone is also responsible for synchronizing reproduction with specific external environment stimuli in order to optimize reproductive success.The aim of this study was to establish the effect of melatonin on zebrafish reproduction.Adult females were daily exposed, via water, to two different doses (100 nM and 1 µM) of melatonin. Melatonin led to an increase of the Gonado Somatic Index (GSI) associated with the increase of eggs production, and the raise of gene and protein levels of vitellogenin (VTG) and estradiol receptor α (ERα) in the liver. The ability of melatonin to increase fecundity was consistent with a significant increase of gene transcription of kiss 1, kiss 2, gnrh3, in the brain, and lh in the pituitary, while in the ovary (in class IIIB follicles), with a significant decrease of two genes codifying for intra-ovarian regulators of premature oocyte maturation, the tgfβ1 and the bmp15. The reduction in the expression of these two genes was concomitant with the increase of lhr and a modulation of mprα and mprβ gene transcription, whose proteins are involved in oocyte maturation. Melatonin also exerted a direct action on follicles as shown by the increase of the oocytes undergoing to germinal vesicle break down (GVBD) and modulated mpr α and β gene expression in the in vitro exposure.These data highlight the effects of melatonin in promoting zebrafish reproduction exerting its effects either in the brain-pituitary and in the gonads.

Highlights

Melatonin, a multifunctional molecule, rhythmically secreted by the pineal gland, plays a major role in several circadian and seasonal processes including animal reproduction [1,2,3,4,5].Reproduction is regulated by the neuroendocrine system: the reciprocal control of the brain and the endocrine system [6] contributes to adapting reproduction to environmental changes
The lh gene transcription was increased by both melatonin doses but only the higher ones induced a significant increase (Fig. 1)
Effects of melatonin on follicle growth These results show that complex ovarian follicle development can be modulated by exogenous melatonin, since Gonado Somatic Index (GSI) was significantly higher in fish exposed to the higher dose of melatonin (Fig. 2)

Summary

Introduction

Reproduction is regulated by the neuroendocrine system: the reciprocal control of the brain and the endocrine system [6] contributes to adapting reproduction to environmental changes. In this regard, melatonin receptors have been found to be localized on the suprachiasmatic nuclei in the brain and in the pars tuberalis of the pituitary [7,8] as well as in the ovary [9,10] suggesting multiple melatonin sites of action in the modulation of reproduction. The control of reproduction by melatonin is species-specific; in seasonal breeders, it is the duration of the daily melatonin secretion rather than its amplitude that encodes day length information [11,12]. Little information is currently available on the role of melatonin on zebrafish, a species that in laboratory conditions behaves as daily spawner with asynchronous ovaries [15]

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