Melatonin foliar application promoted anthocyanin accumulation boosted reactive oxygen scavenging by upregulating the key enzymes activities and genes expression of anthocyanin biosynthesis pathway in green and purple mustard under vanadium stress

Abstract

Heavy metal vanadium (V) is poisonous to both plants and animals. Plant resistance to heavy metals stress can be conferred by the multifunctional signaling molecule melatonin, although the underlying mechanisms are still largely understood. We elucidated the significant contribution of the secondary metabolite anthocyanin to the enhancement of V stress tolerance by melatonin in both green and purple mustard genotypes. The effects of excessive V were leaf yellowing, slowed development, decreased photosynthetic activity, and an increase in the buildup of reactive oxygen species and malondialdehyde in both mustard genotypes. It interesting to note that during V stress, key anthocyanin biosynthetic pathway enzymes activities and leaf anthocyanin concentration increased while exogenous melatonin applied as foliar further boosted activities of key anthocyanin biosynthetic pathway enzymes and leaf anthocyanin concentration in both green and purple mustards. Furthermore, the application of exogenous melatonin increased the levels of endogenous melatonin and mitigated V toxicity. Conversely, the suppression of melatonin intensified V-induced phytotoxicity and delayed the accumulation of anthocyanin by downregulating the transcript levels of essential structural genes. Under V stress conditions, the genes expression related to anthocyanin production, antioxidant enzymes activities, and stress resistance was significantly higher in purple mustard than green mustard genotype. Additionally, melatonin decreased V deposition in roots and leaves. The results recommended that melatonin foliar application to mustard plants inhibited the availability of V to plant and promote V stress tolerance.