Abstract
Changes in melanosome protein composition duringBufo bufo development have been investigated. The number of bands in SDS-PAGE varies from stage 7 (sixth cleavage) to stage 19 (heart beat), with a minimum at stages 17 (tail bud) and 19 (heart beat). At least eleven bands appear to be present throughout stages examined. The most relevant difference observed is that three protein bands of 40, 66 and 115 Kdal are expressed only at pre-neurular stages, when only maternal melanosomes are present in the embryo. Moreover, 5,6-dihydroxyindole oxidase activity has been evidenced in the gels, by specific staining. The activity appears to comigrate with tyrosinase.
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