Melanoma antigen-encoding gene-1 expression in invasive gastric carcinoma: correlation with stage of disease.

Abstract

A human melanoma antigen-encoding gene-1, MAGE-1 gene, may be linked to the neoplastic transformation. In the present study, we extended this association with human gastric carcinomas. Specifically, we focused on the relationship between MAGE-1 gene expression and the histologic stage of gastric carcinoma. We used a reverse transcription-polymerase chain reaction assay (RT-PCR) to analyze the expression of the MAGE-1 gene in 38 endoscopic biopsy specimens from gastric carcinomas. We also studied the relationship between the expression of MAGE-1 gene and the genetic expression of several tumor invasion-related factors, including 72 kD type IV collagenase (MMP2), urokinase-type plasminogen activator (uPA), platelet-derived growth factor A (PDGF-A), and vascular endothelial growth factor (VEGF). Eleven of the 38 tumor samples (28.9%) expressed the MAGE-1 gene. MAGE-1 gene expression was present only in two of the 38 adjacent nontumor samples (5.3%). MAGE-1 gene expression in the 38 tumor samples was significantly correlated with the histological stage of disease (P = 0.0008), especially with the depth of histologically confirmed tumor invasion (t1 vs. t2 or greater, P = 0.00048). The expression of MAGE-1 gene correlated with the expression of MMP2 (P = 0.0064), uPA (P = 0.0390), and PDGF-A (P = 0.00018). These data suggest that the MAGE-1 gene may be activated in gastric carcinomas during periods of their development or invasion. In addition, a relationship between MAGE-1 gene expression and expression of invasion-related factors has been demonstrated.