Abstract
Background and Aim:The genetic improvement of cattle through livestock section is based on quantitative, qualitative, and molecular characteristics. This study examined polymorphisms of the melanocortin-4 receptor (MC4R) and leptin genes as a reference for the selection of superior breeds in Madrasin cattle.Materials and Methods:The leptin and MC4R genes of Madrasin cattle were amplified using polymerase chain reaction (PCR); then, restriction fragment length polymorphism of the leptin gene was performed using the restriction enzyme BsaA1, at site 2793 with ACGT point position.Results:The leptin gene was divided into three bands, namely, AA with one fragment (522 bp), CG with two fragments (441 bp and 81 bp), and AG with three fragments (522 bp, 441 bp, and 81 bp). The MCR-4 gene was divided into three bands, namely, 493 bp, 318 bp, and 175 bp.Conclusion:The MC4R and leptin genes can act as molecular markers for growth traits in Madrasin cattle and can be used to genetically optimize and improve growth. The GG allele of the MC4R gene and the AA allele of the leptin gene can be used in Madrasin cattle.
Highlights
Because of its great variety of plant and animal species, including cattle genetic resources, Indonesia has very high biodiversity
The melanocortin-4 receptor (MC4R) and leptin genes can act as molecular markers for growth traits in Madrasin cattle and can be used to genetically optimize and improve growth
The GG allele of the MC4R gene and the AA allele of the leptin gene can be used in Madrasin cattle
Summary
Because of its great variety of plant and animal species, including cattle genetic resources, Indonesia has very high biodiversity (mega biodiversity). In several areas on Madura Island, artificial insemination is being used aggressively to cross local and Limousine cattle to produce offspring known as Limura; this community is known for crossing local and exotic breeds in hopes of producing highly productive calves [1,2]. Melanocortin-4 receptor (MC4R) is the primary gene responsible for regulating food intake and energy balance. The Creative Commons Public Domain Dedication waiver (http:// creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. This study examined polymorphisms of the melanocortin-4 receptor (MC4R) and leptin genes as a reference for the selection of superior breeds in Madrasin cattle
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