Melanins of Inonotus Obliquus: Bifidogenic and Antioxidant Properties.

Marina A Burmasova,Aidana A Utebaeva,Maria A Sysoeva,Elena V Sysoeva

doi:10.3390/biom9060248

Marina A Burmasova, Aidana A Utebaeva + Show 2 more

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https://doi.org/10.3390/biom9060248

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Journal: Biomolecules	Publication Date: Jun 24, 2019
Citations: 24	License type: CC BY 4.0

Affiliation: Kazan State Technological University

Abstract

Extracts and melanins from Inonotus obliquus are widely used in medicine due to their high antioxidant properties. This study is dedicated to define the influence of the physicochemical and antioxidant properties of Inonotus obliquus melanins and their bifidogenic effects on Bifidobacterium bifidum 1 and Bifidobacterium animalis subsp. lactis. For this purpose, melanins precipitated from Inonotus obliquus aqueous extracts, obtained by a few methods, and separated melanin fractions by organic solvents were used. For the melanin physicochemical properties analysis spectrophotometry, electron paramagnetic resonance (EPR) spectroscopy and dynamic light scattering methods were applied. Melanins and their fractions difference in particle size and charge, antioxidant properties, and redox potential were revealed. It was shown that the redox potential, the size of melanin particles and the z-potential had maximum influence on bifidobacteria growth. The greatest activating effect on bifidobacteria was established by using melanin isolated from aqueous microwave extracts in concentrations of 10−13, 10−10, 10−5 g/cm3. The use of this melanin with antioxidant activity 0.67 ± 0.06 mg/g (expressed as ascorbic acid equivalent), and with redox potential −5.51 ± 2.22 mV as a prebiotic allowed the growth of Bifidobacterium bifidum 1 s to increase by 1.4 times in comparison with ascorbic acid by 24 h of cultivation.

Highlights

IntroductionMuch attention is attracted to the melanins from higher fungi and polypores in antioxidant applications [1]
In modern pharmaceutical chemistry, much attention is attracted to the melanins from higher fungi and polypores in antioxidant applications [1]
Freeze-dried strains of Bifidobacteria B. bifidum 1 and B. animalis subsp. lactis were applied in the study for the melanin bifidogenic effect assay

Summary

Introduction

Much attention is attracted to the melanins from higher fungi and polypores in antioxidant applications [1]. The polypore, Inonotus obliquus ( known as chaga) is a widely used medicinal raw material. Extracts from this species are administered in gastro-intestinal tract diseases and complex anti-cancer treatments [2]. I. obliquus melanins form particles with a size of 400 nm or smaller. They possess paramagnetic properties (EPR signal with a g-factor equal to 2.0044, width of 5.7, and paramagnetic center concentration of 7.4 × 1017 spin/g [5]), and antioxidant activity up to 60 kC/100 g of melanin [6]

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