Abstract
The paper applies MEKC technique to simultaneously carry out quantitative analysis on dairy product’s melamine (Mel) and 5-hydroxymethyl furfural (HMF) bi-component. The quantization analytic experiment starts with a systematic research on operating voltage, electrophoretic buffer solution gravity and optimum pH value, which further influences additives’ concentration as well as other factors, and detects 10 kinds of practical samples on the basis of defined optimum conditions. Detection result indicates that both Mel and HMF showed peaks in 16min, simultaneously acquiring two indicator parameters in one analytical test, This test verifies applicability and scientific validity of MEKC technical approach in dairy product’s Mel and HMF contents detection, which can play an effective role in providing rapid and reliable daily monitoring support for dairy industry.
Highlights
Dairy product contains rich amino acids, which possess many benefits for consumers
This paper presents one detection approach based on micelle electro kinetic capillary electrophoresis (MEKC) technique for the purpose of providing a convenient method for dairy product quantization detection analysis
For optimization of Mel and hydroxymethyl furfural (HMF) detection wavelengths, capillary electrophoresis apparatus diode array detector was adopted to carry out full wavelength scanning at a scope of 190-400nm, by obtaining Mel and HMF ultraviolet absorption spectrograms
Summary
If dairy products exceed the concentration of Mel, it can generate negative effects on consumers [1]. Han Le et al [3] (2013) applied MEKC-DAD approach simultaneously determining different batches of Scutellaria laterifolia and its processed products such as baicalin, scutellaria laterifolia element, wogonin, oroxylin A and apigenin’s contents. Thereby, MEKC technique application feature is able to simultaneously detect the contents of various kinds of materials as compared to the current widely used gas chromatographymass spectrometry and liquid chromatography tandem mass spectrometry. It is capable of overcoming high experiment expending, complicated operational process, long time-consuming as well as other drawbacks. This research paper introduced MEKC technique for detecting Mel and HMF contents in dairy products in the hope of making an initial effort in the improvement and reformation of dairy product monitoring technique
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