Abstract
Oocyte cryopreservation remains a challenge due to its susceptibility to freeze-thaw damage, including disruption of the meiotic spindles and dispersion of chromosomes. There have been conflicting reports as to whether vitrification is more effective than traditional slow-freezing method. The objective of this study is to evaluate and compare the meiotic spindles and chromosome alignment in frozen-thawed oocytes following vitrification and sodium-depleted slow freezing method.
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