Abstract
The kinetics of meiotic chromatin modifications and the function of lipidome in the oocytes that have completed their in vivo or in vitro growth phase after a prolonged culture period have been analyzed in order to improve the efficiency of a technology for extracorporeal maturation of porcine donor-derived oocytes. It is revealed that a prolonged period (up to 50 h) of culturing in the Sage Cleavage medium (CooperSurgical, United States) with the addition of 5% Serum Protein Substitute supplement (Cooper Surgical, United States) and 10 IU human chorionic gonadotropin can provoke destruction of chromatin of BCB+-positive and BCB–-negative oocytes (76 vs. 50%, P < 0.01). The age-associated porcine oocyte processes are usually followed by changes in lipid droplet morphology, since the granules tend to transform into clusters. A high percent of BCB–-negative oocytes containing normal chromatin in metaphase 2 can indicate the capability of oocytes that have not completed their in vivo growth phases to complete it in vitro with the subsequent meiotic maturation. The produced data may be used to model the maturation systems for porcine female gametes that have not completed their in vivo growth phases.
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