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Abstract
Testicular fragments are treated by hypophases (bidistillated water or KCl solutions) during 2-8 h. at 37°; the cells are spread on the surface of sheet grids covered with formvar plus collodion films; interesting stages are selected under LM before observation under EM.Leptotene shows single axes connected with numerous fibers. During zygotene the axes pair two by two and the synaptonemal complex is gradually formed. Pachytene is the most frequent stage, the SC shows the typical tripartite configuration; DNase treatment removes most of the chromatin fibers, but the tripartite structure persists; pronase treatment removes the central element and the transverse fibers, and induces a relaxing of the fibers. During pachytene and diplotene, the chromomeres are clearly individualized, numerous fibers connect the chromosomes with the nuclear envolope; inter-chromosomal fibers join the bivalents. At diakinesis and metaphase, chromosomes are highly condensed and no parts of SC were observed.The above results are compared to those obtained by other water spreading techniques.
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