Meigs’ Syndrome

Abstract

The aims of the present study were to establish the background levels of 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodGuo), 8-oxo-7,8-dihydroguanosine (8-oxoGuo), 8-oxo-7,8-dihydroguanine (8-oxoGua) among a group of healthy Italian children, and to evaluate the contribution of some potential interfering/confounding factors to the urinary (u) levels of these biomarkers of oxidised guanine derivatives.The levels of 8-oxodGuo, 8-oxoGuo, 8-oxoGua, and u-cotinine in urine samples from 159 healthy children (5–11 years) recruited in a cross-sectional study were measured via liquid chromatography–tandem mass spectrometry. Data regarding the anthropometric and life-style characteristics of the participants were obtained from questionnaires.The 5th–95th percentiles of the levels of 8-oxodGuo, 8-oxoGuo, and 8-oxoGua for all children were 2.4–13.9, 3.8–19.9 and 5.4–79.5 μg/L and 2.9–12.6, 4.8–15.2, and 5.1–93.4 μg/g creatinine, respectively.Significant correlations were found between the level of 8-oxoGuo and that of 8-oxoGua and 8-oxodGuo but not between the level of 8-oxoGua and that of 8-oxodGuo in all children and in both the male and female subgroups.Multiple linear regression analyses revealed the independent effect of the investigated variables on 8-oxodGuo, 8-oxoGuo, and 8-oxoGua. u-Creatinine was the most significant predictor of the urinary excretion of both 8-oxoGuo and 8-oxodGuo, age displayed a significant positive independent effect on the level of 8-oxoGuo, whereas the weight status according to the BMI was negatively associated with the level of 8-oxodGuo. None of the chosen independent variables influenced the levels of 8-oxoGua.