Meglumine Cyclic Adenylate Improves Coagulation Abnormalities in Endotoxemic Rats

Abstract

Background: Sepsis and endotoxemia are linked to the concurrent activation of inflammatory and hemostatic pathways, which contribute to organ dysfunction and death. Meglumine cyclic adenylate (MCA) has been found to inhibit NF-κB activity during endotoxemia in rats. This study aims to investigate the effect of MCA on coagulation abnormalities in endotoxemic rats.Methods: Seventy-two male SD rats were randomly divided into three groups (n = 24 per group): Normal saline group (NS), group LPS, and group MCA. Endotoxemia was induced by LPS 10 mg/kg injected via the femoral vein in the LPS and MCA groups. MCA 2mg/kg was injected 20 min before LPS injection in group MCA via the femoral vein. An equal volume of normal saline was given to NS and LPS groups. Prothrombin time (PT), partial activated thromboplastin time (APTT), platelet count, cAMP, plasminogen activator inhibitor- 1(PAl-1), D-dimer, TNF-α, antithrombin III (ATIII), t-PA and Tissue factor (TF) were assessed before (baseline) and 2, 4, 6 h after LPS injection.Results: After LPS administration, PT and APTT were prolonged, ATIII concentrations, cAMP, and platelet count were significantly decreased, while plasma PAI-1, D-dimer, t-PA, TNF-α, and TF levels were significantly increased compared to group NS. However, these alterations induced by LPS were inhibited by MCA treatment.Conclusion: MCA amelio-rates coagulation abnormalities induced by LPS in endotoxemic rats.