Abstract
Using the culturomics method, two strains were isolated, identified and characterized following the taxonogenomics concept. Megasphaera vaginalis sp. nov. strain Marseille-P4512 (= CSURP4512) and Anaerococcus vaginimassiliensis sp. nov. strain Marseille-P4857 (= CSURP4857) were isolated from the vagina of a French woman. The phylogenic tree, phenotypic criteria and genomic analysis described here clearly show that these two bacteria are different from previously known bacterial species with standing in nomenclature and new members of Firmicutes phylum.
Highlights
Healthy vaginal microbiota is a complex dynamic ecosystem, mainly dominated by Lactobacillus spp. and classified into five community state types (CST) depending on the following majority species: CST I (Lactobacillus crispatus), CST II (Lactobacillus gasseri), CST III (Lactobacillus iners) and CST V (Lactobacillus jensenii) [1,2]
We report here the description of two new designated species, Megasphaera vaginalis sp. nov. strain Marseille-P4857 and Anaerococcus vaginimassiliensis sp. nov. strain MarseilleP4512, belonging to the Firmicutes phylum
Strain identification and phylogenetic analysis Attempts to identify the strains cultivated on blood agar by mass spectrometry failed, indicating that these isolates were not known from the MALDI-TOF database
Summary
Healthy vaginal microbiota is a complex dynamic ecosystem, mainly dominated by Lactobacillus spp. and classified into five community state types (CST) depending on the following majority species: CST I (Lactobacillus crispatus), CST II (Lactobacillus gasseri), CST III (Lactobacillus iners) and CST V (Lactobacillus jensenii) [1,2]. These beneficial bacteria are the first line of defence against vaginal pathogens through competition and production of inhibitory compounds [3,4]. Among the 13 Anaerococcus species validly published, six were isolated from vaginal discharge or ovarian abscess samples: Anaerococcus hydrogenalis, Anaerococcus lactolyticus, Anaerococcus vaginalis, Anaerococcus prevotii, Anaerococcus tetradius and Anaerococcus provencensis [16,18]
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