Abstract
Megagametophyte development in barley (Hordeum vulgare 'Atsel') was studied using Nomarski-interference optics and transmission electron microscopy. Stages described include the functional megaspore to cell wall formation. Aspects of the transition from the free nuclear stage of the embryo sac to the cellular embryo sac indicate involvement of elongate cell plates associated with clusters of microtubules. Initial cell walls among micropylar and chalazal nuclei are composed of beads derived from dictyosome vesicles. Fusion of growing cell plates occurs, especially within the antipodal apparatus.
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