Abstract
A semi‐automated method to determine pK a values of drugs and chemicals spectrophotometrically is described. The method uses the capabilities of a HPLC with a diode array detector (DAD) as a flow injection apparatus and a binary solvent delivery system. The advantages of this method are low sample consumption (less than 0.1 mg compound needed), high sample throughput, easy set up, high sensitivity, and low cost, precision within a 0.2 unit. Experimental pK a values obtained for over thirty model compounds are consistent with literature values.
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