Medium Term Conservation of Several Carnation Accessions Via in Vitro Culture

Kurniawan Budiarto,Budi Marwoto

doi:10.31258/jnat.13.2.174-177

Abstract

Sufficient genetic diversity is important in carnation breeding program. In vivo conservation of carnation germplasmis considered inefficient due to some technical and economical aspects. In vitro conservation was then, expectedto overcome the limitation of in vivo method. The research was conducted to find out the proper media for medium-term in vitro conservation of several carnation accessions in low temperature storage. A complete factorialexperiment with 25 replications was designed to accomplish the combination of two factors. The first factor wassix commercial carnation cultivars, namely Pink Maladi, Orange Triumph, Opera, Tundra, Yellow Liberty and PradoReffit. The second factor was the conservation media i.e. 1⁄2MS + DMSO 3% and 1⁄2MS + 3% DMSO + 3% sucrose andcontrol (MS 0+3% sucrose). The results showed that in vitro conservation of carnation in low temperature weresuccessfully conducted using 1⁄2MS+3% DMSO and 1⁄2MS+3% DMSO+3% sucrose without significant variation in allaccessions tested up to 10 and 12 months respectively. The increase of death plantlets, however, was detected onthe media of 1⁄2MS+3% DMSO after 6 months storage with significant decrease in viability hereafter. The existenceof sucrose in DMSO media induced root formation and plantlet resistance to low temperature storage.

Highlights

Carnation (Dianthus carryophyllus) is one of famous ornamental cut flowers in the world
The growth inhibitors for in vitro conservation were usually divided into osmotic pressure, nutrient modification and growth regulators
The rooted cuttings of carnation accessions were collected from commercial nurseries in West Java

Summary

INTRODUCTION

Carnation (Dianthus carryophyllus) is one of famous ornamental cut flowers in the world It belongs to dicotyledonous plants and member of Caryophyllaceae family. In vitro conservation was considered to be one promising methods in preserving the collection and reduced the limitation of in vivo conservation. This method was usually conducted using cell growth inhibitor and protectant. The growth inhibitors for in vitro conservation were usually divided into osmotic pressure, nutrient modification and growth regulators. Considering the possibility of the use of nutrient modification and cell protectant, a medium term in vitro conservation of carnation was studied. The objective of the experiment was to find out the in vitro conservation method by media modification and supplemental DMSO for carnation in low temperature storage

MATERIALS AND METHODS

RESULT

CONCLUSIONS