Abstract
The increased demand for natural polymers for diverse industrial applications in last years has led to a renovated interest in exopolysaccharides (EPS) production by microorganisms. Ecological roles of EPS especially for those isolated from extreme habitats such as hypersaline ponds have been reported in several studies. A moderate halophilic strain producing exopolysaccharide (EPS) has been isolated earlier. However, the EPS production of the strain (2 g/L) was relatively low to exploit it at the industrial level. In the present work, the strain was identified as Bacillus zhangzhouensis and named as BZ 16 and the optimal medium was studied to boost the EPS production. Maximum EPS production was obtained in medium with 125 g/L sucrose, 30 g/L yeast extract. Phosphate source inhibited EPS production even if it increased the growth. Under optimal medium composition, EPS was produced at 12.37 g/L, which was 6 times greater than the production yield achievable without optimizing conditions.
Highlights
Halophilic bacteria are widely present in the hypersaline environment and have been recognized as precious sources of novel biomolecules including exopolysaccharides [1]
It is proved that the yields and compositions of the EPS are impacted by culturing conditions, especially when using various carbon sources [18,19] which yield the necessary energy for cell growth and components for EPS production [20,21]
The maximum EPS production and increased B.zhangzhouensis BZ 16 cell biomass,when sucrose was used as the sole carbon source, was in good agreement with previous findings, suggesting that sucrose is the most profitable carbon source for EPS mass production [20,23]. Mannitol occurs in a wide range of living organisms, where it fulfills different physiological roles
Summary
Halophilic bacteria are widely present in the hypersaline environment and have been recognized as precious sources of novel biomolecules including exopolysaccharides [1] These exopolysaccharides (EPS) are thought to play important role in protecting against desiccation, toxic compounds, bacteriophages, osmotic stress, at the same time as allowing attachment with substrates, adhesion to solid surfaces and facilitating the development of biofilms [2]. EPS have stirred great interest among biotechnologists because of their physico-chemical characteristics allowing their commercial application in wide range of fields such as medicine, pharmacy, food, cosmetics and the petroleum industry [2,4,5,6] In addition to their contribution in the biogeochemical cycling in the ocean [7], today, polysaccharides represent a possible ecological substitute of petroleum industry, as their sources are renewable and not pollutant. They are biodegradable, not toxic and their chemical degradation can produce biofuel with other molecules, which are able to form industrial polymers and fine chemicals [8]
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