MEDIUM CONSTITUENTS AS AFFECTING THE GROWTH OF Cordyline terminalis CV. ATOOM IN VITRO. a- EFFECT OF NATURAL SOURCES AND MS SALT STRENGTHS.

Abstract

Aseptic cultures (shoots) of Cordyline terminalis grown in vitro were used as source of explants in this study. The medium of all experiments of this study contained I mg/ L BA, 30-g/ L sucrose and 2 g/ L gelrite. Full strength of MS observed the highest values of all parameters (shoot number/explant, colour intensity/ explant and growth vigor/ explant) compared to ½ and ¼ MS strengths. The same treatment (full MS) significantly showed the highest response in the same measured parameters compared to all examined sources of natural nutrients (white corn, bean, chick pea, lentil, banana and date, each alone at 60 g/ L) especially at the second record after 2 months. MS strengths were examined in combinations with banana and date each alone at 60 g/ L. Interestingly, the treatment of banana (60g/ L) and ½ MS significantly showed similar high response as that of full strength of MS in all parameters mentioned before, either with using one or three explants in cultures. Mixing banana and date each at 30g/ L in combinations with the used strengths of MS, observed lower records in all parameters compared to full MS alone. According to the results of this study and from the economic point of view, recommendation can be raised as to use the medium contained banana (60g/ L) + ½ strength of MS to regenerate cordyline in vitro.