Mediterranien cuttlefish sepia officinalis squid ink is cytotoxic but does not inhibit Glioblastoma U87 tumor cells proliferation, with high nutritional values of edible viscera

Abstract

This study aims to identify nutritional values of some viscera’s cuttlefish Sepia officinalis commonly edible in Tunisia (Nidamental gland, hepatopancreas, male gonad, squid ink) and to evaluate the role of cuttlefish ink, on viability and proliferation of human glioblastoma cells U87.Biochemical analysis shows that solid and liquid viscera are rich in water, in Protein and in total Ash. They contain less fat and Carbohydrates. Energy values are substantial. Sex and Cooking over low have no significant effect on these nutritional values. These results lead us to encourage the use of these marine co-products (generally rejected), not only for food but for prevent and reduce the incidence of certain diseases. In fact our results show also a cytotoxic effect of cuttlefish ink at increasing doses on human cells cancer Glioblastoma U87 for an incubation time of 5 hours with a concentration higher than 20μg/ml. The IC50 value is 25μg/ml which may be due to tyrosinase, responsible for the cephalopod ink toxic. Supernatant of cuttlefish ink Sepia offinalis does not block proliferation of cancer cells U87 during 4 days of incubation. Further analyses are warranted and necessary to substantiate and complete our findings such as the study of the effect of this ink in adhesion and migration of Glioblastoma cells U87.