Abstract
Introduction. Medicinal plant raw materials are one of the most important sources of herbal remedies used both for the prevention and treatment of a number of diseases [1, 2]. Even with the development of modern science and chemistry, medicinal plant raw materials are widely used in both folk and official medicine. The advantage of medicinal plants is their wide range of biological activity, low toxicity and the possibility of long-term use without significant side effects. Human economic activity has a noticeable negative effect on the condition of wild-growing plants: their stocks are decreasing, and some species disappear altogether. Today, given the high level of development of industry and agriculture, the procurement of raw materials for wild medicinal plants is not always possible. Information on the quantitative assessment of the raw material base of wild medicinal plants in the Middle Urals, the content of biologically active substances in medicinal plant raw materials is partially outdated, which determines the need for their systemic resource study and chemical-pharmacognostic study.Aim. Comprehensive assessment of the state of populations of wild medicinal plants in the Middle Urals.Materials and methods. Determination of stocks of raw materials of the studied species of medicinal plants was carried out on specific thickets according to the generally accepted method. The authenticity of the raw materials was established by a macroscopic method when collecting raw material samples. In the course of the study, samples of medicinal plant materials of 5 types were prepared. The determination and assessment of the main indicators of the good quality of medicinal plant raw materials (the content of active and extractive substances, moisture in the mass upon drying, total ash and ash insoluble in a 10% solution of hydrochloric acid) was carried out according to the methods and requirements of the State Pharmacopoeia of the Russian Federation XIV edition. In the raw materials Artemisiae absinthii herba and Leonuri herba, the amount of extractives was determined by the gravimetric method. The quantitative assessment of the content of essential oil in the samples of Origani vulgaris herba and Tanaceti vulgaris flores was carried out by the method of hydrodistillation. To determine the quantitative content of the sum of flavonoids in Hyperici herba, Artemisiae absinthii herba, Leonuri herba and the sum of flavonoids and phenolcarboxylic acids in Tanaceti vulgaris flores, a spectrophotometric method was used.Results and discussion. In the course of resource and phytochemical studies of representatives of the medicinal flora of the Middle Urals, a comprehensive assessment of the state of populations of wild medicinal plants – sources of medicinal plant raw materials (Origani vulgaris herba, Hyperici herba, Tanaceti vulgaris flores, Artemisiae absinthii herba and Leonuri herba) was carried out. The results are included in the electronic inventory of wild medicinal plants of the Middle Urals.Conclusion. The conducted complex of studies will allow updating information about the medicinal flora of the Middle Urals in order to use raw materials for the creation of medicines.
Highlights
Medicinal plant raw materials are one of the most important sources of herbal remedies used both for the prevention and treatment of a number of diseases [1, 2]
Even with the development of modern science and chemistry, medicinal plant raw materials are widely used in both folk and official medicine
Information on the quantitative assessment of the raw material base of wild medicinal plants in the Middle Urals, the content of biologically active substances in medicinal plant raw materials is partially outdated, which determines the need for their systemic resource study and chemical-pharmacognostic study
Summary
В качестве объекта исследования использовались лекарственные растения Origanum vulgare L., Hypericum perforatum L., Hypericum maculatum Crantz., Tanacetum vulgare L., Artemisia absinthium L., Leonurus quinquelobatus Gilib., и лекарственное растительное сырье Origani vulgaris herba, Hyperici herba, Tanaceti vulgaris flores, Artemisiae absinthii herba и Leonuri herba, заготовленное летом 2019–2021 гг. на территории Пермского края и Свердловской области, после чего сырьё было подвергнуто первичной обработке и высушено воздушно-теневым способом. Определение запасов сырья изучаемых видов ЛР проводили на конкретных зарослях по общепринятой методике. Подлинность сырья устанавливали макроскопически при сборе образцов сырья. Определение и оценку основных показателей доброкачественности лекарственного растительного сырья (содержание действующих и экстрактивных веществ, влажности в массе при высушивании, золы общей и золы, нерастворимой в 10 % растворе кислоты хлористоводородной) проводили по методикам и требованиям Государственной Фармакопеи РФ XIV издания. В сырье пустырника и полыни горькой проводили определение суммы экстрактивных веществ гравиметрическим методом [11]. Количественную оценку содержания эфирного масла в образцах травы душицы и цветков пижмы проводили методом гидродистилляции [11]. Для определения количественного содержания суммы флавоноидов в траве зверобоя и цветках пижмы использовали спектрофотометрический метод [11]. Оценку микробиологической чистоты сырья проводили по методике ОФС.1.2.4.0002.15 «Микробиологическая чистота» [11]. Статистическую обработку результатов проводили по общепринятым методикам
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