Abstract
Bromelain is obtained from Ananas comosus and is of significant clinical interest. Many studies have demonstrated bromelain's ability to treat immune-mediated conditions including reduction of platelet aggregation, angina pectoris and surgical trauma. ROS elimination were evaluated using a variety of anti-oxidation effect tests containing DPPH assay, ABTS+ radical scavenging assay, hydrogen peroxide scavenging assay. Western blotting analysis and RT-PCR were used to investigate an anti-inflammatory agent in LPS-induced RAW cells. Treatment with bromelain exhibited significant scavenging abilities on superoxide radicals compared with control. As results of analyzing iNOS and COX-2 band appearance according to bromelain treatment, it inhibited the band expression dose-dependently. Also bromelain inhibits the phosphorylated-MAP kinases signaling pathways. Furthermore, it reduced c-Fos, phosphorylation of c-Jun and c-Jun. According to the results, it is demonstrated that bromelain of Ananas comosus extract have significant anti-inflammatory ability as well as antioxidant ability.
Highlights
Bromelain has been studied for its anti-thrombotic and anti-metastatic properties
The purpose of this study was to assess the antioxidant ability of a preparation of bromelain from Ananas comosus using in vitro free radical scavenging assays
The primers used for amplification of Inducible nitric oxide synthase (iNOS), COX-2, Tumor necrosis factor α (TNF-α) and β-actin were purchased from Bionics, Inc (Emeryville, CA, USA)
Summary
The beneficial effects of bromelain have been suggested or proven in a variety of inflammatory diseases and models. These include immunologically mediated arteriosclerosis in rat aortic allografts [3], allergic encephalomyelitis (EAE) model of multiple sclerosis [4], collagen-induced arthritis in the rat. [5] In study, bromelain had an efficacy similar to standard anti-inflammatory drugs. [8] The beneficial therapeutic effects of bromelain have been suggested or proven in preclinical animal models of inflammation as well as in human inflammatory bowel disease and arthritis. Bromelain has been shown to have beneficial effects in a variety of inflammatory diseases in both humans and mice. Bromelain has been shown to have beneficial effects in a variety of inflammatory diseases in both humans and mice. [9]
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