Abstract
ABSTRACTObjective:Testicular torsion (TT) refers to rotation of the testis and twisting of the spermatic cord. TT results in ischemia-reperfusion (I/R) injury involving increased oxidative stress, inflammation and apoptosis, and can even lead to infertility. The aim of this study was to investigate the effect of ozone therapy on testicular damage due to I/R injury in an experimental torsion model.Materials and Methods:24 male Sprague-Dawley rats were divided into 3 groups; shamoperated, torsion/detorsion (T/D), and T/D+ozone. Ozone (1mg/kg) was injected intraperitoneally 120 minutes before detorsion and for the following 24h. Blood and tissue samples were collected at the end of 24h. Johnsen score, ischemia modified albumin (IMA), total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) levels were determined.Results:Levels of IMA, TOS, OSI, and histopathological scores increased in the serum/tissue of the rats in the experimental T/D group. Serum IMA, TOS, and OSI levels and tissue histopathological scores were lower in the rats treated with ozone compared with the T/D group.Conclusion:Our study results suggest that ozone therapy may exhibit beneficial effects on both biochemical and histopathological findings. Clinical trials are now necessary to confirm this.
Highlights
Testicular torsion (TT) results from the impairment of testicular and epididymal blood flow following rotation of the testicular spermatic cord and blood vessels [1]
Serum ischemia modified albumin (IMA), total oxidant status (TOS), and oxidative stress index (OSI) levels were significantly higher in the T/D group compared to the control group (p=0.006, 0.0001, and 0.0001, respectively), but the levels of these parameters were significantly reduced by medical ozone therapy (p=0.0001 for all parameters)
Our results show that medical ozone therapy significantly reduced IMA, TOS, and OSI values that normally rise in a TT model
Summary
Testicular torsion (TT) results from the impairment of testicular and epididymal blood flow following rotation of the testicular spermatic cord and blood vessels [1]. Progressive interruption of testicular venous flow occurs Time intervals between torsion and detorsion and degree of spermatic cord torsion are the main factors determining the severity of ibju | Protective effect of medical ozone in TT model testicular injury. In association with this loss of function, a decrease in fertility occurs in the ipsilateral testis, with testicular atrophy occuring in severe cases. An increase in mitochondrial Ca2+ activates various proteases and phospholipases, and cell lysis occur These changes resulting from I/R injury trigger biochemical mechanisms, such as oxidative stress and inflammation [3]. Despite all the researches that have been performed, no additional therapeutic methods with easy clinical adaptation and proven utility have to date been obtained
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