Abstract
Medicagenic acid (MA) content was studied in four Mexican, one Spanish and five USA cultivars of alfalfa (Medicago sativa L) using high-performance liquid chromatography (HPLC) and two bioassays: Trichoderma viride inhibition and ichthyotoxic activity. The MA contents (g kg−1), measured through HPLC, for the 10 varieties were: Valenciana=0·165; Inia 76=0·115; Puebla 76=0·097; Synthetic I=0·042; Pierce=0·031; Maxidor=0·027; Condor=0·024; Synthetic II=0·023; Sundor=0·023; NK-819=0·013. These data confirm the influence of the variety in MA contents. Fungitoxic and ichthyotoxic assays showed that the Spanish cultivar (Valenciana) presented the highest toxicity. The biological activity of the ten varieties was strongly correlated (r=0·97) to medicagenic acid content measured through HPLC. ©1997 SCI
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