Abstract
AbstractTetrathiafulvalene serves as a useful mediator for glucose oxidase in its application in second‐generation enzyme electrodes for monitoring glucose. Among its useful characteristics are a low redox potential, fast homogeneous electron exchange kinetics with glucose oxidase, high stability, and low solubility in aqueous solutions. The fabrication and characterization of tetrathiafulvalene‐glucose oxidase enzyme electrodes in flow‐injected and stopped‐flow analysis is described. One of the conclusions is that the homogeneous rate constant for tetrathiafulvalene is higher than that of dimethyl ferrocene. Tetrathiafulvalene is, however, more susceptible to pH variations. The characteristics and advantages of the mediated enzyme/wall‐jet amperometric detection are described.
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