Abstract
Background Chinese Hamster Ovary (CHO) derived cells are the most commonly used cell lines for the production of biopharmaceuticals. We developed a plug and play method to optimize the growth medium for a given CHO production cell line. The experiment consists of a Mixture Design of Experiments (DOE) approach using different basal media to select the optimized formulations for a specific CHO cell line. The package for media optimization consists of different base media together with the ambr15 high throughput bioreactor and integrated MODDE DoE software. Methods and results A suspension adapted CHO DG44 cell line expressing a monoclonal antibody was used for the experiments. Media mixes composed of differing concentrations of nutrients were used in a mixture DOE with 20 different media and 3 center points. All conditions were inoculated in the ambr at the same starting density of 2.0xE5 cells/mL. Dissolved oxygen, pH, and temperature control were maintained throughout the batch process. Each bioreactor was sampled for cell count and viability. Metabolites as well as IgG titers were measured. Peak Viable Cell Density (VCD), Doubling Time (DT), and IgG titer results were
Highlights
Chinese Hamster Ovary (CHO) derived cells are the most commonly used cell lines for the production of biopharmaceuticals
We developed a plug and play method to optimize the growth medium for a given CHO production cell line
The experiment consists of a Mixture Design of Experiments (DOE) approach using different basal media to select the optimized formulations for a specific CHO cell line
Summary
Chinese Hamster Ovary (CHO) derived cells are the most commonly used cell lines for the production of biopharmaceuticals. We developed a plug and play method to optimize the growth medium for a given CHO production cell line. The experiment consists of a Mixture Design of Experiments (DOE) approach using different basal media to select the optimized formulations for a specific CHO cell line. The package for media optimization consists of different base media together with the ambr high throughput bioreactor and integrated MODDE DoE software
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