Media optimization, extraction, purification and characterization of exopolysaccharide from Fusarium proliferatum: A novel source bioactive polysaccharide

Abstract

Polysaccharides from fungal source have been proved to be effective in different plethora of biomedical fields. The present study was carried out on exopolysaccharide (EPS) production by Fusarium proliferatum, considered novel fungus as no reports on its EPS production capability has been reported yet. The main objective of this study was to optimize media for better EPS production extracellularly under submerged culture conditions. Among all malt extract broth was selected as favorable medium for EPS yielding 81.4 ± 0.48 mg/l. Optimization revealed 9 days of incubation period, pH 6.0 and temperature 25 °C best for the growth and EPS yield. The medium 12 out of sixteen media was selected as best for the EPS yield which was ensured by various permutation and combinations of chemical factors. The optimized medium includes: malt extract medium (basal medium), xylose 4%, glucose 4%, tryptophan 0.1%, olive oil 3%, Tween 80 0.2%, vitamin C 0.2%, K2HPO4 0.2%, CaCl2 0.5%, pH 6.0, incubation period of 9 days at temperature 25 °C yielding 4067 ± 153.08 mg/l of EPS. HPTLC, FTIR and LCMS analysis exhibited the monomeric composition of maltose, fructose, xylose, galactose, glucose, raffinose and sorbose with functional group of –OH, C=O, C–O–C, C–O, –C–H, COO– with glycosidic likanges and suggested the production of glycan by these fungi. Owing to the existing reports of anti-cancerous property of β-Glycan an in-silico investigation was carried out to which indicatedits higher reactivity and lower binding affinityin comparison to imatinib, an oral chemotherapy drug to treat cancer.