Abstract
Problem statement: Some component of fermentation medium showed to reduce the bacterial production of hydrogen. Approach: Reinforced clostridium medium is a selected medium for Clostridium species. Reformulation this medium regarding hydrogen production may focus on such medium composition that enhance or reduce the bacterial productivity. The optimum pH and temperature for hydrogen production were at initial pH of 7.0 and 30°C. Results: The results show that both nitrogen source and its concentration affected biomass growth as well as H2 yield. Yeast extract at concentration of 13 gL-1 was the best organic nitrogen source and resulted in hydrogen yield (YP/S) of 308 mL g-1 glucose utilized with biomass concentration of 1.1 gL-1, hydrogen yield per biomass (YP/X) of 280 mL g-1 L-1, biomass per substrate utilized (YX/S) of 0.22 and produced hydrogen in gram per gram of glucose utilized (YH2/S) of 0.0275. C/N of 70 enhanced the YP/S from 308 mL g-1 to 350 mL g-1 glucose utilized with biomass concentration of 1.22 gL-1, YP/X of 287 mL g-1 L-1, YX/S of 0.244 and (YH2/S) of 0.03125. In the absence of sodium chloride and sodium acetate further enhanced YP/S from 350 mL g-1 glucose utilized to 391 mL g-1 glucose utilized with maximum hydrogen productivity of 77.5 mL L-1 h-1, whereas RCM medium gave the highest hydrogen productivity of 63.5 mL L-1h-1. Results also show that Sodium Chloride and Sodium Acetate in the medium adversely affect growth. Removal of both components from the medium enhanced the biomass concentration from 1.22-1.34 gL-1, YP/X of 254 mL g-1 L-1, YX/S of 0.268 and (YH2/S) of 0.0349. Conclusion: The medium an improved containing (glucose 5 gL-1, Yeast extract gL-1, L-Cystine. HCl 1 gL-1 and Bacteriological agar 0.5 gL-1), was able to enhance the hydrogen productivity.
Highlights
Fossil fuel, the major global energy resource, causes global atmospheric pollution problems during combustion
Effect of organic nitrogen source on hydrogen production: The results shown in Table 1 indicated that
The results illustrated that organic nitrogen was better than inorganic source for hydrogen production with maximum hydrogen yield of 308 mLg−1 glucose utilized (b) with that the results shown in Fig. 2c showed that organic nitrogen was better for bacterial growth than
Summary
The major global energy resource, causes global atmospheric pollution problems during combustion. Hydrogen is produced mainly from fossil fuels, but biohydrogen can be produced from biomass Biohydrogen production processes, including fermentation, are more environmentally friendly and less energy intensive compared to thermo-chemical and electrochemical processes in hydrogen production[1]. The first step to consider is an equation based on the stoichiometry for growth and product formation[2] A fermentation process consists of a number of operations and stages for which a whole family of inter-related media must be developed. There is no requirement for final product formation the medium may be designed purely to satisfy the growth requirement of the organism. In order to obtain rapid growth in seed and final stages, the medium must contain sources of energy, carbon, nitrogen and phosphate, trace metal and any specific growth factors which the organism itself cannot manufacture.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
