Abstract
Mechanosensitive (MS) ion channels commonly play a role of transducers converting mechanical stimuli into electrical or chemical signaling, thus allowing the cell to regulate its behavior in response to changing environment conditions. MS channels participate in sensation of sound and orientation in inner ear (hair cells), in touch sensation and in osmoregulation of bacteria. Structure of bacterial MS channels of large (MscL) and small (MscS) conductance has been recently solved at atomic resolution, stimulating various structural and functional studies. In this work author presents series of experiments enhancing an understanding of mechanosensation in bacteria. In Chapter 2 author performs cysteine cross-linking experiments suggesting asymmetric gating pattern of Tb-MscL ion channel. Chapters 3 and 4 establish a possibility of successful synthesis of fully functional Tb- and Ec-MscL proteins displaying a phenotype identical to recombinant channels. Studies in Chapter 5 and Appendix 1 extend the resolution of single-channel patch clamping technique, and describe a fine structure of MS channel gating by collecting and characterizing intersubstate transitions.
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