Abstract
A mixture of L-[3,3-2H2]lysine plus L-[2-13C]lysine is converted by purified lysine 2,3-aminomutase into β-lysine. The di-N-phthaloyl methyl ester derivative of the resultant β-lysine shows a geminally deuterium-coupled enriched 13C n.m.r. signal indicating intermolecular transfer of the migrating 3-pro-R hydrogen of the α-lysine. L-[3-13C]Lysine has been synthesized. A mixture of L-[3,3-2H2]lysine plus L-[3-13C]lysine is similarly converted into β-lysine. Its di-N-phthaloyl methyl ester derivative shows a β-shifted enriched 13C n.m.r. signal for C-3, but no geminally coupled enriched C-3 signal. The latter experiment rules out any intermolecular exchange of the non-migrating 3-pro-S hydrogen atom of α-lysine. Various mechanistic possibilities for the intermolecular hydrogen transfer process are discussed.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
