Abstract
Phyllanthus amarus Schum. & Thonn. (Phyllanthaceae) is a medicinal plant that is commonly used to treat diseases such as asthma, diabetes, and anemia. This study aimed to examine the antiallergic activity of P. amarus extract and its compounds. The antiallergic activity was determined by measuring the concentration of allergy markers release from rat basophilic leukemia (RBL-2H3) cells with ketotifen fumarate as the positive control. As a result, P. amarus did not stabilize mast cell degranulation but exhibited antihistamine activity. The antihistamine activity was evaluated by conducting a competition radioligand binding assay on the histamine 1 receptor (H1R). Four compounds were identified from the high performance liquid chromatography (HPLC) analysis which were phyllanthin (1), hypophyllanthin (2), niranthin (3), and corilagin (4). To gain insights into the binding interactions of the most active compound hypophyllanthin (2), molecular docking was conducted and found that hypophyllanthin (2) exhibited favorable binding in the H1R binding site. In conclusion, P. amarus and hypophyllanthin (2) could potentially exhibit antiallergic activity by preventing the activation of the H1 receptor.
Highlights
The Phyllanthaceae family, which was formerly included in the Euphorbiaceae family, is consists of 59 genera, which is considered as a major family in Angiosperm
The reaction is mediated by antibody immunoglobulin E (IgE) [24]
Histamine increases the permeability of blood vessels and attracts leukocytes to the allergic site, whereas no specific involvement of beta-hexosaminidase is reported in allergic reactions [27,28]
Summary
The Phyllanthaceae family, which was formerly included in the Euphorbiaceae family, is consists of 59 genera, which is considered as a major family in Angiosperm (flowering plants). It is the plant family for the Phyllanthus genus [1,2]. Among the Phyllanthaceae family, the Phyllanthus genus, which consists of 884 species, was widely reported for its traditional uses and biological activities. Some of the species were reported to have significant antiallergic activity. Leaves extract exhibited antianaphylactic activity through its evaluation using allergic pleurisy, passive cutaneous anaphylaxis, and passive paw anaphylaxis on the rat [4].
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
