Abstract
CVD are a major human health problem across the globe. Acute myocardial infarction (MI) is a leading cause of cardiovascular diseases. Studies indicate that micro RNAs (miRs) play an important role in the development of cardiovascular disease. The present finding was used to explore the role of miR-24 andmiR-34expression in rat MI. Sprague–Dawley rats were used for developing a MI (MI) model. The pathology of MI was confirmed by histological analysis. The miRs expression was determined by qRT-PCR. Immunoblotting was applied to determine protein expression ofTGF-β1 and E2F3. The histological analysis revealed the development of MI in rats. The evaluation of miR-24 and miR-34 expression showed that miR-24 and miR-34 were upregulated in rats with MI. Moreover, the expression of these microRNAs increased considerably during the progression of MI from 1st to the 5th week. Target Scan analysis showed that miR-34 and miR-24 exert their effects by targeting transforming growth factor beta 1 (TGF-β1) and E2F transcription factor 3 (E2F3), respectively. Therefore, the expression of both E2F3 and TGF-β1 was also examined by Western blot analysis and qRT-PCR. Results revealed that the expression of TGF-β1 and E2F3 were downregulated after the 3rd and 4th week of infarction. The results suggest that miR-34 and miR-24 could be useful biomarkers for the diagnosis, progression and treatment of cardiovascular diseases.
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