Mechanisms of reduced luteal sensitivity to prostaglandin F 2α during maternal recognition of pregnancy in ewes

Abstract

During maternal recognition of pregnancy, the conceptus stimulates endometrial secretion of PGF 2α and PGE 2. However, PGF 2α is less effective in causing luteal regression in pregnant than in non-pregnant ewes. Experiments were conducted to elucidate mechanisms for reduced luteal sensitivity to PGF 2α during maternal recognition of pregnancy. Corpora lutea (CL) were collected from pregnant and non-pregnant ewes 0, 4, or 12 h following treatment with PGF 2α on day 12 after estrus. Luteal PTGHS2 mRNA did not differ due to PGF 2α or pregnancy status. Luteal PTGES mRNA was reduced in both pregnant and non-pregnant ewes after PGF 2α treatment; while, luteal PTGFS mRNA was reduced 4 h after PGF 2α in pregnant, but not non-pregnant ewes. The result was a greater ratio of PTGES to PTGFS mRNA in pregnant ewes. Luteal mRNA for HPGD did not differ between pregnant and non-pregnant ewes on day 12. Luteal END1 mRNA was reduced in pregnant as compared to non-pregnant ewes prior to PGF 2α challenge. Luteal END1 mRNA was increased after PGF 2α in pregnant and non-pregnant ewes; however, ECE1 mRNA was reduced 4 h after PGF 2α in pregnant, but not non-pregnant ewes. The in vitro conversion of PGF 2α to PGFM was greater in CL of pregnant than non-pregnant ewes at day 14. Luteal conversion of PGF 2α to PGFM appears to be regulated post-transcriptionally. During maternal recognition of pregnancy, mechanisms of reduced luteal sensitivity to PGF 2α may include a shift in prostaglandin production to the luteotropin PGE 2, a reduction of ECE1 mRNA, and increased catabolism of PGF 2α.