Mechanisms of potassium absorption by higher plant roots

Abstract

Potassium, as a plant macronutrient, is accumulated in plant cells from relatively dilute soil solutions and is indispensable for many vital processes. Studies characterising potassium uptake by roots stretch back over many decades. However, it is only with the introduction of modern electrophysiological and molecular techniques that investigations have been possible at a molecular level. Such approaches have confirmed the existence of discrete high and low affinity uptake systems at the root plasma membrane and have greatly enhanced our understanding of the underlying molecular nature of these uptake systems.High affinity K+ uptake from micromolar external K+ levels is coupled to H+ transport as demonstrated independently by patch clamping of single root protoplasts and by studying the transport system after expression in Xenopus oocytes. The measured coupling ratio between the two ions is 1:1 and is sufficient to account for an accumulation ratio in excess of 106, a value which encompasses experimental observations on K+ accumulation.Low affinity K+ uptake activates at relatively high external K+ levels in the millimolar range and is ‘passive’ i.e. down the electrochemical gradient for potassium. In two higher plant species single cell inward potassium currents have been identified which are associated with low affinity potassium uptake. Furthermore, specific ion channels which underlie these potassium influxes and form a major constituent of the low affinity potassium uptake pathway have been identified and characterised.