Mechanisms of peroxynitrite-induced [Ca2+]i increase in single neuronal cell

Abstract

The mechanism of peroxynitrite (ONOO−)-induced [ca2+]i increase in single MN9D cell (Dopaminergic neuroblastoma cell line) was studied by using Fura-2 microfluorometric technique. The results show that ONOO− caused a rapid increase of [Ca2+]i when ONOO− was puffed to the cell. Removing Ca2+ from the bath or using calcium channel antagonist (CdCl2, Nifedipine) greatly inhibited the [Ca2+]i increase induced by ONOO−1, suggesting that the opening of L-Ca2+ channel makes a great contribution to the [Ca2+]i increase. The effect of sulfhydryl reductive agent (DTT) on ONOO−-induced [Ca2+]i increase suggests that ONOO−-activating L-Ca2+ channel is partly related to its oxidative speciality.