Abstract
This chapter reviews the optics of pulsed laser microbeams and the use of basic instrumentation to provide pulsed laser microbeam capabilities within a microscope platform. Moreover, we review the principal mechanisms by which laser microbeams produce microsurgical effects in cellular targets. We discuss the principal photothermal, photomechanical, and photochemical damage mechanisms as well as their relationship to critical laser microbeam parameters, including wavelength, pulse duration, and numerical aperture. We relate this understanding of damage mechanisms to laser microbeam applications reported in the literature.
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