Mechanisms of inorganic carbon acquisition in twoEuglenaspecies

Abstract

The mechanism of inorganic carbon uptake was examined in Euglena gracilis Klebs. and the acidophilic species Euglena mutabilis Schmitz. Both species, whether grown in acidic (pH 3.5) or alkaline (pH 7.5) media lack external carbonic anhydrase. Acid-grown E. gracilis was shown to have no capacity for bicarbonate transport, but transport was induced on transfer to alkaline medium (pH 7.5) in the light over a period of 8 h. In contrast, acid-grown E. mutabilis appears to have no capacity for bicarbonate transport even at neutral pH. The overall internal pH of the cells was determined by equilibration with14C-labelled benzoic acid over the pH range 3.5–5.0 and with14C-labelled 5,5-dimethyloxazolidine-2,4-dione over the range pH 5.5–7.5. The acidophilic species maintains an internal pH range of 6.6–6.8 in an external pH range of 3.5–5.5, whereas the acid-tolerant species E. gracilis maintains a neutral internal pH in an external pH range of 3.5–7.5. Measurement, by mass spectrometry, of the fluxes of CO2and O2in photosynthesizing cells at pH 3.5 demonstrated a rapid uptake of CO2by both species that was completely blocked by iodoacetamide, an inhibitor of CO2fixation. Uptake of CO2by E. gracilis, grown at pH 7.5, was not completely inhibited by iodoacetamide and O2evolution was sustained when the cells reached the CO2compensation concentration, indicating a direct uptake of bicarbonate. These data indicate that the acidophilic species, E. mutabilis, takes up CO2by diffusion, whereas the acid-tolerant species, E. gracilis, takes up CO2by diffusion at acid pH levels but has some capacity for active bicarbonate uptake when grown at alkaline pH levels.Key words: acidophilic alga, acidotolerant alga, bicarbonate uptake, CO2uptake, Euglena gracilis, Euglena mutabilis, internal pH.