Abstract
Study DesignTo investigate the specific mechanisms by which Golgi phosphoprotein 3 (GOLPH3) affects the progression of gastric cancer and to explore its clinical significance.MethodsImmunohistochemical analysis was used to evaluate the correlations between GOLPH3, phosphorylated mTOR (p-mTOR), phosphorylated Akt (p-Akt), phosphorylated p70S6 (p-p70S6), phosphorylated 4E-BP1 (p-4E-BP1) and the clinicopathological features of gastric cancer. The mRNA expression levels of GOLPH3, mTOR, Akt, p70S6 and 4E-BP1 in gastric cancer, carcinoma-adjacent and paired normal tissue were analyzed using RT-PCR. Western blotting was used to determine the protein expression of GOLPH3, p-mTOR, p-Akt, p-p70S6 and p-4E-BP1 in tissues.ResultsHigh expression protein levels of GOLPH3, p-AKT, p-mTOR, p70S6, p-4E-BP1 were positively associated with histological grade (p<0.05), depth of invasion (p<0.05), distant metastasis (p<0.05) and lymph node involvement (p<0.05). Compared with carcinoma-adjacent and paired normal tissues, the mRNA expression levels of GOLPH3, AKT, mTOR, p70S6 and 4EBP1 in gastric cancer tissues were significantly higher. The protein expression levels of GOLPH3, p-AKT, p-mTOR, p-p70S6 and p-4E-BP1 in gastric cancer tissues were also significantly higher than in carcinoma-adjacent and paired normal tissues. A strong positive correlation was observed between GOLPH3, p-mTOR, p-p70S6 and p-4EBP1 expression (r = 0.410, 0.303 and 0.276, respectively, p<0.05), but no significant correlation between the expression of GOLPH3 and p-Akt was observed.ConclusionsThe GOPLH3 expression level is highly correlated with Akt/mTOR signaling in human gastric cancer samples. GOLPH3 combined with Akt/mTOR signaling activation may play an important role in the development, differentiation, invasion and metastasis of gastric cancer.
Highlights
Global statistics show that gastric cancer (GC) is the fourth most common cancer in men and the fifth most common cancer in women, the mortality from gastric cancer ranks second only to lung cancer [1]
The GOPLH3 expression level is highly correlated with Akt/mammalian target of rapamycin (mTOR) signaling in human gastric cancer samples
Golgi phosphoprotein 3 (GOLPH3) combined with Akt/mTOR signaling activation may play an important role in the development, differentiation, invasion and metastasis of gastric cancer
Summary
Global statistics show that gastric cancer (GC) is the fourth most common cancer in men and the fifth most common cancer in women, the mortality from gastric cancer ranks second only to lung cancer [1]. The global incidence of gastric cancer has been declining since World War II [2]. In developing nations, including China, the morbidity and mortality of gastric cancer have remained high. Gastric cancer treatment has improved in recent years, but most patients are still diagnosed with advanced gastric cancer, frequently including invasion and distant metastasis, leading to an unsatisfactory treatment effect rate. Thereafter, the sections were soaked in the citrate antigenic retrieval buffer, heated in an autoclave, and allowed to cool to room temperature. After the sections were washed in PBS three times for five minutes, 3%
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