Abstract
AbstractThe development of biofilms in contact with flowing liquids was monitored in a biofouling culture apparatus which contained aluminium tubes and which was inoculated with Pseudomonas fluorescens. Tests were carried out in which, alternately, the nutrient feed or the bacterial supply was stopped. Results showed that once the surface is colonised, the predominant mechanism for biofilm development was growth within the film. Maximum biofilm development occurred at liquid flow velocities around 1 m s−1. Rapid development occurred even in the presence of very small amounts of nutrient.
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